DNA Replikasyon çatalında 3' - 5' yönünde replikasyon işlemi DNA Polimeraz III tarafından rahatça gerçekleştirilir. Fakat diğer iplikçik 5' - 3' yönündedir ve DNA Polimeraz III enzimi bu yönde yapısı bakımından replikasyon gerçekleştiremez. Bu sebeple Primaz enzimi belli aralıklarla anlamsız primer(Resimde 5.kısımda çıkıntılı yer) deniler kısımları o iplikçik üzerine yerleştir. Ve böylece kısa aralıklarla 3' - 5' yönleri elde edilmiş olunur. Bu aralıklar tekrar DNA Polimeraz III enzimi ile doldurulur. Geriye kalan anlamsız primerler DNA Polimeraz I enzimi tarafından kaldırılır (Resimde 6.kısım) ve kopyalama o bölgelere tekrar yapılır. Bu işlemler sonrasında aralıklı kopyalamadan dolayı yeni oluşan iplikçikler arasında kopukluklar (aralıklar) kalmış olur (Resimde 7.kısım). Bu aralıkların birleştirilmesi işlemini DNA Ligaz enzimi yapar.
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