Eskimiş sıvı kültürlerinden, durma veya ölme döneminde olanlardan, katı besiyerlerine ekim yapılırsa başlıca iki tür koloni tipine rastlanır. Bunlar S-tipi ve R-tipi kolonilerdir.
S-tipi: Yuvarlak, düzgün, parlak, pürüzsüz ve konveks R-tipi: Pürüzlü, mat ve düzensiz
Bu koloniler dışında başta tip kolonilerin oluşmasıda muhtemeldir (intermedier koloniler ve mukoid-M).
Çok faza pasaj yapılmış veya eskimiş kültürlerde R-tipi koloni variasyonlarına rastlamak genellikle mümkündür. S-tipi -> R-tipi variasyonları için ortama Lityum Klorid (Lithium chloride) veya anti-S immunserum katılması yeterlidir. Fakat R-tipi -> S-tipi variasyonları bu kadar kolay değildir. Bu variasyonu sağlamak için, duyarlı laboratuvar hayvanlarında pasaj yapılması gereklidir. S-tipi -> R-tipi variasyonlarında bazı değişlikler olur.
S-tipler
R-tipler
1- Patojeniktir
1- Patojenesi azalmış veya tamamen kaybolmuştur
2- Genellikle kapsüllüdürler
2- Kapsüllü değildirler
3- Koloniler düzgün, yuvarlarlak,parlak ve konvekstir
3- Koloniler pürüzlü, mat ve düzensizdir
4- Sıvı ortamlarda homojen ürerler
4- Sıvı besiyerlerinde granüllü ürerler ve dipte tortu yaparlar
5- Fizyolojik su ile homojen bir süspansiyon oluştururlar
5- Fizyolojik su ile granüler bir süspansiyon oluştururlar
Mikroorganizmalar, penisilin veya diğer baskılayıcı kimyasallar ile katı besiyeri ortamında üretilirse, normalinden çok fazla değişikliğe sahip L-formlu koloni oluşutur (Ortası papillalı koloniler). Bu baskılar eğer mikropların üzerinden kaldırılırsa, bu mikroorganizmalar normal koloni ve bireysel formlarına geri dönerler.
· Yazar admin
- Şubat 17 2008 13:31:58 ·
375 Yorum ·
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