Bir nükleusun varlığı, ökaryotik ve prokaryotik canlıları ayıran en temel özelliktir. Prokaryot canlılarda çekirdek yani nükleus bulunmaz. Çekirdek hücrenin kontrol merkezidir ve genetik bilgiyi içinde barındırır. Ve replikasyon, transkripsiyon işlemleri bu yerde gerçekleşir. Translasyon ise nükleus dışında yani sitoplazmada gerçekleşir.
Prokaryotik canlılarda mRNA proteine çevrilirken transkripsiyon devam etmektedir. Fakat ökaryotik canlılarda transkripsiyondan sonra bir çok işlem yapılır ve mRNA nükleus dışında en son olarak proteine çevrilir(translasyon). Ökaryotik canlılarda traskripsiyon sonrası mRNA düzenleme işlemleri yine nükleus tarafından kontrol edilir.
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